真菌诱导子对悬浮培养西洋参细胞的生理效应

THE PHYSIOLOGICAL EFFECTS OF FUNGAL ELICITORS ON THE PANAX QUINQUEFOLIUM CELLS IN SUSPENSION CULTURE

报道了不同真菌诱导子对悬浮培养的西洋参（Ｐａｎａｘｑｕｉｎｑｕｅｆｏｌｉｕｍ）细胞生长、皂甙和多糖合成，以及细胞内和培养液中过氧化物酶活性的生理效应。悬浮培养的西洋参细胞经刺盘孢菌（Ｃｏｌｌｅｔｏｔｒｉｃｈｕｍｎｉｃｏｌｔｉａｎａｅ）丝体诱导子处理后，总皂甙产率可由对照的２９６ｍｇ／Ｌ增加到６７９ｍｇ／Ｌ（约占细胞干重的（１６．３％），比对照提高约１．３倍，而且总皂甙的８５％排放在培养液中；经黑曲霉（Ａｓｐｅｒｇｉｌｌｕｓｎｉｇｒａｎ）诱导子处理后，细胞多糖含量可达到１１．７９％（细胞干重），比对照增加１倍多。初步纯化的刺盘孢菌丝体诱导子和尖孢镰刀菌（Ｆｕｓｕｒｉｕｍｏｘｙｓｐｏｒｕｍ）滤液诱导子在诱导处理前期能明显促进西洋参细胞生长，同时细胞内及培养液中过氧化物酶活性显著增加；随时间延长，细胞生长和酶活性逐步受到抑制。

The physiological effects of fungal elicitors on the cell growth ,the biosynthesis of saponin and polysaccharide and the peroxidase activity of Panax quinquefolium cells in suspension culture was reported. After treated with Colletotrichum nicoltianae mycelia elicitor , the productivity of total saponin of Panax quinquefolium cells in suspension culture went up from 296 mg / L to 679 mg /L(about 16. 3 % DW) ,increasing 130% over the control, and about 85% of total saponin were excreted into the medium. The polysaccharide content of Panax quinquefolium cells treated with Aspergillus nigran elicitor increased to 11.79%,up more than 100% over the control.The cell growth of P. quinquefolium was firstly promoted by adding C.nicoltianae mycelia and Fusurium oxsporum filtration elicitors, meanwhile ,the peroxidase activity in the cells and in the medium remarkable rose, and then both of the cell growth and the POD activity were inhibited.