系统性红斑狼疮和类风湿性关节炎T细胞异常调节与免疫功能紊乱的研究

Research on the relationship between abnormal regulation of T lymphocytes and disturbance of immune function in systemic lupus erythematosus and rheumatoid arthritis

目的分析系统性红斑狼疮(SLE)和类风湿性关节炎(RA)外周血T细胞亚群表面共刺激信号分子表达,探讨人类SLE和RA中T细胞免疫紊乱状态。方法采用流式细胞技术检测SLE、RA患者外周血T细胞亚群表面CD28、CD152、诱导性共刺激因子(ICOS)、CD154、CD30和CD95分子表达。结果与健康对照组比较,SLE和RA患者组分别为CD3+CD8+T细胞和CD3+CD4+T细胞增加(P<0.05);SLE患者CD4+T细胞和CD8+T细胞上CD28和CD152分子表达率均增加,ICOS分子表达减少(P均<0.05),CD154和CD30分子表达率均下降;RA患者2类T细胞亚群上CD28分子表达均降低,CD152分子表达率均增加(P均<0.05),ICOS分子表达率无明显变化,CD154和CD30分子表达率分别增加或减少;SLE和RA患者CD4+T细胞和CD8+T细胞上CD95分子表达率均明显增加。结论SLE和RA有不同的外周T细胞亚群平衡失控;T细胞的异常活化受复杂的细胞共刺激信号网络分子调控。

Objective To analyse the expression of peripheral blood T lymphocyte subsets and costimulators on them in patients with systemic lupus erythematosus（SLE） and rheumatoid arthritis（RA） and discuss the disturbance of T lymphocytes in human SLE and RA. Methods Apply flow eytometry to determine the expressions of costimulators CD28, CD152, ICOS, CD154, CD30 and CD95 on T lymphocyte subsets in patients with SLE and RA. Results Compared with healthy control, percentage of CD8^＋ T cell and CD4^＋ T cell increased significantly in SLE and in RA respectively （ P 〈 0.05 ）. Expressions of CD28 and CD152 on CD4^＋ T cells and CD8^＋ T cells in SLE increased strikingly, that of ICOS on them decreased strikingly （P 〈0.05） as well as the dropping of CD154 and CD30; In RA expressions of CD28 on T lymphocyte subsets decreased significantly, that of CD152 increased（ P 〈 0.05 ）, that of ICSO on T cell subsets in SLE was similar to that in healthy control（ P 〉0.05 ） ; That of CD154 and CD30 on CD4^＋ T cells or CD8 ^＋ T cells increased or decreased respectively. Expression of CD95 on CD4^＋ T cells and CD8^＋ T cells in SLE and RA were more than that in healthy control. Conclusions Patients with SLE and RA have different characteristics of peripheral blood T lymphocyte subsets disturbances and the abnormal activation of T cells is regulated by the complex costimulatory net.