EB病毒感染的系统性红斑狼疮患者中BYRF1基因和BcLF1基因的表达与系统性红斑狼疮分期的关系

The relationship between the expression of the gene BYRF1 and BcLF1 in Epstein-Barr Virus positive syetemic lupus erythematosus patients and progress of the disease.

目的探讨EB病毒(EBV)感染的系统性红斑狼疮(SLE)患者EBV基因BYRF1和BcLF1表达与SLE的关系。方法应用聚合酶链反应(PCR)-Southern杂交技术检测44例SLE患者和43名正常对照外周血单个核细胞中EB病毒(EBV)特异性DNA片段,反向(RT)-PCR检测EBV特异性DNA片段阳性患者病毒潜伏期基因BYRF1和增殖期基因BcLF1的表达。结果SLE患者组EBV特异性DNA片段BamH-ΙW阳性率为72.73%(32/44),明显高于正常对照组6.98%(3/43)(2χ=39.177 9,P=0)。32例EBV BamH-ΙW片段阳性标本中有20例BYRF1mRNA阳性,其中活动期患者9例,稳定期患者11例,活动期和稳定期患者阳性率的差异无显著性(P=0.290 7)。EBV增殖期基因BcLF1 mRNA 16例阳性,其中活动期患者14例,稳定期患者2例,活动期和稳定期患者之间的差异有显著性(P=0.000 2)。结论BYRF1 mRNA和BcLF1 mRNA的表达可能在一定程度上参与SLE的发病和病程进展。

Objective To explore the correlation between systemic lupus erythematosus （SLE） and the expression of the gene BYRF1 and gene BcLF1 of Epstein-Barr virus （EBV） positive SLE patients. Methods 44 SLE patients and 43 normal controls matched in age and sex were tested for EBV specific DNA fragment in peripheral blood monocytes by polymerase chain reaction （PCR） -Southern blotting. RT-PCR was performed to detect the expression of the gene BYRF1 in latent period and the gene BcLF1 in proliferated period for EBV specific DNA fragment positive patients. Results 72.73% of the SLE patients were EBV specific fragment positive （32/44）, obviously above the normal individuals 6.98% （3/43） （X^2 = 39. 177 9 ,P =0） ,of the 32 SLE EBV specific DNA fragment positive samples,20 were positive in BYRF1 mRNA, among which 9 were active and 11 were stable. Statistics analysis showed no significant difference between the active and stable groups（P =0. 290 7）. 16 were positive in the gene BcLF1 mRNA for proliferated EBV,among which 14 were active and 2 were stable. The difference between the active and stable patients was statistically significant （P =0. 000 2）. Conclusions The expressions of BYRF1 mRNA and BcLF1 mRNA may play a certain role in the SLE developing and the cause process.