摘要
目的观察制首乌对脑内灌流6-羟基多巴胺(6-OHDA)所致大鼠纹状体细胞外液羟自由基升高的影响,探讨制首乌对脑保护的作用机理。方法用脑内微透析造模,水杨酸捕获羟自由基,高效液相-电化学检测器测定活体脑内羟自由基所形成的2,3二羟基苯甲酸(2,3-DHBA)和2,5二羟基苯甲酸(2,5-DH-BA)。结果6-OHDA脑内灌流后,模型组大鼠纹状体细胞外液2,3-DHBA和2,5-DHBA均迅速增高,前者在观察全程中一直显著高于对照组(P<0.01);后者部分时间点显著高于对照组(P<0.05或P<0.01);制首乌组的2,3-DHBA有5个时点显著低于模型组(P<0.05或P<0.01)。结论制首乌对6-OHDA脑内灌流引起的细胞外液羟自由基升高有一定程度的抑制作用,提示其脑保护作用的机理之一与抗氧化有关。
Objective To investigate the effect and mechanism of prepared Radix Polygoni Multiflori (RPM) on the elevation of extraeellular hydroxyl radical in striatum of rats induced by intraeerebral perfusion of 6-hydroxy dopamine (6-OHDA). Methods Cerebral mierodialysis was used to establish the model. Hydroxyl radical was captured by salicylic acid, and 2,3-dihydroxy benzyl acid (2,3-DHBA) and 2,5-dihydroxy benzyl acid (2,5-DHBA) formed by hydroxyl radical in vital brain were measured by high performance liquid chromatography-electrochemical detector (HPLC- ED). Results After perfusion of 6-OHDA in brain of rats, the levels of 2,3-DHBA and 2,5-DHBA in the model group increased rapidly. The former was higher during the whole course of observation (P 〈 0.01 ), while the latter was higher at most time points than that in the control group (P〈0.05 or P〈0.01). The level of 2,3-DHBA in the RPM group was lower than that in the model group at 5 time points (P 〈 0.05, P 〈 0.01 ). Conclusion RPM could inhibit the elevating of extracellular hydroxyl radical in striatum of rats induced by intracerebral perfusion of 6-OHDA, indicating one of the brain protective mechanisms of RPM may be related to its anti-oxidation effect.
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2006年第3期236-239,共4页
Chinese Journal of Integrated Traditional and Western Medicine
基金
国家自然科学基金资助项目(No.30371824)
关键词
制首乌
脑内微透析
6-羟基多巴胺
羟自由基
水杨酸捕获
prepared Radix Polygoni multiflori
cerebral microdialysis
hydroxyl radieal
6-hydroxy dopamine
capture by salicylic acid