河北省虫媒病毒分离鉴定

Isolation and identification of arboviruses in Hebei Province

目的 研究河北省蚊虫携带虫媒病毒情况。方法 在蚊虫孳生地采集标本液氯保存，按照20—30只／组经无菌处理后研磨，研磨液离心后接种C6／36细胞，连续三代观察致细胞病变情况；对细胞病变阳性的分离物，进行血清学和分子生物学鉴定。结果 在河北省涉县两个村共采集蚊虫1310只，分46组进行处理，共获得13株阳性分离物，其中两株分离物经间接免疫荧光检测提示为甲病毒属成员，用甲病毒属特异性引物RT-PCR扩增阳性，经核酸序列测定证实该序列与Getah病毒（AY702913．1）同源性最高（98％），初步鉴定为Getah病毒，其他病毒分离物尚在鉴定。结论 本研究从河北省蚊虫标本中分离到Getah病毒，这是我国内陆地区首次分离到该病毒。

Objective To study the arboviruses carried by mosquitoes collected in Hebei Province. Methods Samples were collected from mosquito active sites and stored in liquid nitrogen till use. Pools of 20 to 30 mosquitoes were ground after sterilization, centrifugal supernant was inoculated onto C6/36 cell, cytopathic effect was observed for three sequential passages. Positive isolates were identified by IFA and RT-PCR. Results Totaly 1310 mosquitoes were collected from two villages of She eounty,Hebei province. They were divided into 46 pools and ground respectively. Thirteen postive isolates were obtained. Two isolates reacted with alphaviral antibodies and were amplified by alphaviral primers, nucleotide sequence showed the highest homology （98 % ） to Getah virus （AY702913. 1）,so the two isolates were identified as Getah virus. Conclusion Getah virus was isolated from mosquitoes in Hebei Province. This is the first report of isolating Getah virus from inland of China.