辣椒疫霉菌孢子诱导技术研究

Studies on Methods of Spore Inducing for Phytophthora capsici

通过将辣椒疫霉菌在马铃薯、胡萝卜、玉米粉、燕麦、大豆配制的5种固体培养基上繁殖并进行连续光照培养、连续暗培养、低温4℃、高温40℃和间断光照5种不同处理诱导孢子囊进行比较。结果表明:辣椒疫霉在CA培养基上暗培养,经间歇光照处理,仅培养了9 d孢子囊产量就高达11.05个/视野。持续暗培养的菌丝体未产生孢子囊,持续光培养的菌丝体上孢子囊数量随光照时间的延长而增多。培养基种类影响辣椒疫霉菌菌落形态、生长速度;气生菌丝较发达的培养基上菌丝体产孢量明显大于气生菌丝细弱的培养基;菌丝体生长速度与产孢量之间关系不明显。

Phytophthora capsici was cultured on potato sucrose agar （PSA）, carrot agar （CA）, cornmeal agar （CMA）, oatmeal agar （OMA）, bean agar （BA） these five different mediums and with five different treatments of continual illumination, continual darkness, 4℃, 40℃, disconnected illumina tion respectively, and then compared the spore yield. The results showed that only 9 days culture, the sporangiums, which were induced in CA medium, with disconnected illumination treatment, was up to 11.05 sporangia in every eyeshot. Light was the necessary condition to sporangium formation. Medium types affected P. capsici sporangium yield and colony grow speed and colony shape in some degree. The sporangia which were induced from exuberance mycelia was much more than the weak ories.