小麦秆锈病细胞间隙液的提取及注射技术研究

Studies on the Extracting and Injecting Technique of IWF for Puccinia graminis f.sp.tritici

通过真空渗入离心法提取小麦秆锈病病组织的细胞间隙液,并采用注入法将细胞间隙液注入到健康的小麦细胞,研究了小麦秆锈病细胞间隙液对小麦叶片细胞HR的诱导作用,明确了细胞间隙液的提取和注射技术在小麦秆锈病细胞间隙液研究中利用的可行性。

Primary leaves of wheat infected by the Puccinia graminis f. sp. tritici were detached, infiltrated with redistilled water, and centrifuged to yield intercellular washing fluids （IWF）. IWF were then injected into the healthy wheat leaves. The result showed the induction of IWF to the hypersensitive reaction （HR） on wheat leaves. Two methods were available in the research of IWF.