首乌藤药材质量标准的研究

Study on Quality Standards for Caulis Polygoni Multiflori

目的：建立首乌藤药材的质量标准。方法：采用TLC法对首乌藤药材进行定性鉴别；用高效液相色谱法测定2,3,5,4＇-四羟基二苯乙烯-2-O-β-D-葡萄糖苷的含量。结果：在TLC色谱中首乌藤斑点清晰；2,3,5,4＇-四羟基二苯乙烯-2-O-β-D-葡萄糖苷在0.0309～1.54μg范围呈良好的线性关系，r=0.99965：平均回收率为95．5％，RSD=3．2％。结论：所建立的方法可准确地进行定性、定量检测，可用于首乌藤药材的质量控制。

Objective： To establish the quality standards for Caulis Polygoni Multiflori. Method： The TLC and HPLC methods were used for identifi-cation and quantitative determination of 2,3,5,4＇-Tetrahydroxystilbene-2-O-β-D-glucoside. Result： Caulis polygoni multiflori could be identified by TLC. 2,3,5,4＇-Tetrahydroxystilbene-2-O-β-D-glucoside showed a good linear relationship at a range of 0.0309～1.54μg, r = 0.99965. The average recovery was 95.5%, and RSD was 3.2%. Conclusion： The methods can be used for the quality control of Caulis Polygoni Muhiflori.