摘要
目的:建立首乌藤药材的质量标准。方法:采用TLC法对首乌藤药材进行定性鉴别;用高效液相色谱法测定2,3,5,4'-四羟基二苯乙烯-2-O-β-D-葡萄糖苷的含量。结果:在TLC色谱中首乌藤斑点清晰;2,3,5,4'-四羟基二苯乙烯-2-O-β-D-葡萄糖苷在0.0309~1.54μg范围呈良好的线性关系,r=0.99965:平均回收率为95.5%,RSD=3.2%。结论:所建立的方法可准确地进行定性、定量检测,可用于首乌藤药材的质量控制。
Objective: To establish the quality standards for Caulis Polygoni Multiflori. Method: The TLC and HPLC methods were used for identifi-cation and quantitative determination of 2,3,5,4'-Tetrahydroxystilbene-2-O-β-D-glucoside. Result: Caulis polygoni multiflori could be identified by TLC. 2,3,5,4'-Tetrahydroxystilbene-2-O-β-D-glucoside showed a good linear relationship at a range of 0.0309~1.54μg, r = 0.99965. The average recovery was 95.5%, and RSD was 3.2%. Conclusion: The methods can be used for the quality control of Caulis Polygoni Muhiflori.
出处
《中国实验方剂学杂志》
CAS
2006年第3期7-9,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家科技攻关计划(No:2001BA701A38)