HPLC法测定加拿大紫杉中紫杉醇的含量

Determination of Taxol in Canadian Yew by HPLC

目的：测定加拿大紫杉中紫杉醇的含量。方法：高效液相色谱法（HPLC），Agilent ZOBAX C18色谱柱（250×4.6mm，5μm）；流动相：甲醇-水（65：35），流速：1mL/min，检测波长：227nm。结果：紫杉醇线性范围为0．068～0．34μg，r=0.9998；平均回收率为98．90％，RSD=3.17％。结论：本法操作简便、准确，可作为加拿大紫杉的质量控制方法之一。

Objective： To develop an HPLC method for determination of taxol in Canadian yew. Method： ZOBAX C18 Column was used. The mobile phase was methanol-water （65：35） at a flow rate of 1 mL/min. The detection wavelength was at 227nm. Results： The linear relationship of peak area and injection amount of Taxol was in the range 0.068～0.34μg, r = 0.9998. The average recovery was 98.90% and RSD was 3.17%. Conclusion： This method is simple, accurate and could be used for quality control of Canadian yew.