摘要
目的:观察疡愈涂剂对链脲佐菌素(STZ)引起的糖尿病大鼠伤口细胞增殖及体外对成纤维细胞增殖迁移的作用。方法:实验分为正常组、模型组、疡愈涂剂高、中、低剂量组。除正常组外,大鼠腹腔注射STZ,造成高血糖状态。各组动物复合背部全厚皮切除伤口。分别观察疡愈涂剂对创面愈合时间、愈合率;采用免疫组化法观察细胞核增殖抗原(PCNA)表达;及MTT法和体外伤口法观察疡愈涂剂对糖尿病大鼠创面成纤维细胞的增殖和迁移作用。结果:与模型组相比,不同剂量的疡愈涂剂均能明显缩短STZ诱发糖尿病大鼠复合创伤后创面的愈合时间(分别P<0.01);提高创面愈合率(P<0.05,P<0.01)。创伤3天后疡愈涂剂高剂量组PCNA表达明显高于模型组;中剂量组于5天后开始增高;低剂量组7天后增高;分别P<0.05,P<0.01。体外研究表明疡愈涂剂在29.23μg/mL和58.45μg/mL能促进糖尿病大鼠创面成纤维细胞的增殖和迁移,分别P<0.01。结论:疡愈涂剂可能通过促进糖尿病大鼠创面成纤维细胞的增殖,从而加速创面的愈合。
Objective： To study the effects of YangYuTuJi （YYTJ） on cell proliferation of diabetic rats caused by streptozotocin （STZ）, and its effects on fibroblast proliferation and migration in vitro. Methods： SD male rats except for control group （C） were given 55mg/kg STZ by intraperitoneal, randomly divided into model group（M） and 3 diferent dose groups of YYTJ. A round skin of Ф1.6cm was excised on the back of rats. The healing time and healing rate were observed. Proliferation cell nuclear antigen （PCNA） was studied by immuno-histochemistry assay. Fibroblasts from normal and wounded site of diabetic rat skin were cultured as normal fibroblast （NFB） and diabetic fibroblast（DFB）. MTT assay was used for cell proliferation, and cell wound model in vitro for migration analysis. Results： Three YYTJ groups shorten the healing time of the wound of diabetic rats caused by STZ （P 〈 0.01 respectively）, and increased the healing rate （P 〈 0.05, P 〈 0.01 respectively）. From day 3 the PCNA expression elevated in YYTJ-H group, day 5 in YYTJ-M group and day 7 in YYTJ-L group （P 〈 0.05 and P 〈 0.01 respectively）. In vitro study we found YYTJ at both 29.23μg/mL and 58.45μg/mL promoted DFB proliferation and its migration. Conclusion： It is possible that YYTJ accelerates wound healing by promoting cell proliferation and cell migration.
出处
《中国实验方剂学杂志》
CAS
2006年第3期44-47,共4页
Chinese Journal of Experimental Traditional Medical Formulae
