热休克诱导艾氏腹水癌细胞的抗肿瘤效应

Anti-tumor efficacy derived from heat shocked Ehrlich ascites tumor cell

目的研究热休克后的肿瘤细胞内多种热休克蛋白(HSP)的抗肿瘤效果及其作用机理。方法应用丝裂霉素C(MMC)灭活经43℃热休克2 h或14 h的小鼠艾氏腹水癌细胞(EAC),以此作抗原进行体内肿瘤免疫保护试验及小鼠淋巴细胞体外肿瘤杀伤实验。结果经43℃热休克2 h或14 h的EAC细胞攻击后,小鼠淋巴细胞对EAC细胞杀伤率分别为67.6%、80.6%,与对照组比较均有显著性差异(P<0.05,P<0.01);腹水生成量明显低于对照组(P<0.05)。结论热休克后的EAC细胞内的HSPs表达增多,并随热休克时间的延长而增加,其免疫效果也相应增强与多种HSPs的联合作用有关。

Objective To investigate the immune protective effects of the heat shocked EAC and the effect of anti-tumor by heat shock proteins（HSP） in vitro. Methods Mice were immunized with heat- shocked and MMC-inactivated EAC cells. Immunized with EAC cells which had been treated at 42 ℃ for 2 h or 14 h, then mice were challenged by the same type tumor cells . Results The cytotoxic rote of mouse lymphocytes induced by the heat-shocked EAC cells at 42 ℃ for 14 h or 2 h were 80.6% （P〈0.01）, 67.6% （P〈0.05） respectively, compared with the PBS control group, which were significantly different. Conclusion Immune response induced by heat-shocked EAC cells increases and immune protection can be produced against the same type of tumor attack.