菠棱子提取液对组胺-乙酰胆碱诱发豚鼠咳喘的影响

Effect of the Extract of the Seeds of Spinacia Oleracea on Cough and Asthma in Guinea Pigs Induced by Histamine and Acetylcholine

目的观察菠棱子提取液抗动物咳喘的作用。方法幼年豚鼠随机分成4组,超声雾化法吸入不同制剂。空白对照组吸入0.9%氯化钠溶液;低、高剂量实验组吸入100%,200%菠棱子提取液;阳性对照组吸入0.05%异丙肾上腺素。各组超声雾化吸入0.01%组胺和0.2%乙酰胆碱诱发咳喘,观察各组动物致喘的潜伏期。结果高、低剂量实验组及阳性对照组均可延长组胺/乙酰胆碱诱发豚鼠咳喘的潜伏期,与空白对照组比较差异有极显著性(P<0.01)。结论菠棱子提取液具有对抗组胺/乙酰胆碱诱发豚鼠咳喘作用。

Objective To survey the anti-tussive and antiasthmatic effects of the seeds of Spinacia oleracea（SSOL） in animals. Methods 48 young guinea pigs were randomly divided into 4 equal groups. Animals of all 4 groups were subjected to inhalation of different solutions nebulized by an ultrasonic device ： guinea pigs of the control group inhaled nebulized 0.9 % sodium chloride solution, animals of the low-dose trial group, 100 % extract of SSOL ;those of the high-dose trial group, 200 % extract of SSOL, and guinea pigs of the positive control group, 0.05 % solution of isoprenaline hydrochloride. The inhalation lasted 3 minutes and was followed immediately by inhalation of nebulized solution of 0.01% histamine-0.2 % acetylcholine for 20 s in order to induce asthma. The animals were then kept under observation for 6 min for the attack of asthma. Results The latent period of asthma attack was prolonged both in animals of the high-dose and low-dose trim groups, and the difference was significant（ P 〈 0.01 ） if compared with that in animals of the control group. Conclusion The extract of SSOL was shown to exert antagonistic effect against the cough and asthma induced by histamine-acetylcholine in guinea pigs.