2,4-D和BAP对蒙古冰草幼胚愈伤组织诱导及生长的影响

Influence of 2,4-D and BAP on Callus Induction and Growth of Immature Embryo of Wheatgrass

以蒙古冰草为材料,在离体培养条件下对其幼胚的发育进行了研究。结果表明:蒙古冰草的幼胚在不含任何激素的培养基上能直接萌发,幼胚发芽率因培养基而异。N6+7%蔗糖培养基萌发率最高,达96%;其次为MS+5%蔗糖培养基,萌发率为90%。当幼胚被培养在减半的N6培养基或减半的MS培养基上时,发芽率均显著降低。以上四种培养基均未发生脱分化现象。相反,在含有2,4-二氯苯氧乙酸(2,4-D)的培养基上,蒙古冰草幼胚不同程度地发生了脱分化,并出现了少量再生芽。在继代培养基中,降低2,4-D浓度,附加低浓度6-苄氨基嘌呤(BAP)可以改善蒙古冰草幼胚愈伤组织状态,增加胚性愈伤组织诱导率,从而提高分化率。

The immature embryo development of wheatgrass（Agropyron mongolicurn）was studied in vitro culture. The immature embryo of wheatgrass developed directly into normal plantlets on MS or N6 culture medium without any hormones. The germination rates varied with medium, the germination rate on N6 culture medium was the highest, 96.5% and next MS culture medium, 90%. The germination rates re duced when half N6 or half Ms culture medium was used. No dedifferentiation was observed on above culture medium. In contrast, wheatgrass immature embryo dedifferentiated when 2,4 13 was added to the culture medium. A few plantlets were regenerated from resulting callus. The embryogenic callus frequency and callus differentiation frequency were advanced in MS snlld rnoclium with 0.5mg/L BAP.