对4个不同地理种群褶皱臂尾轮虫遗传多样性的RAPD分析

RAPD analysis of genetic diversity in four different populations of rotifer Brachionus plicatilis

采用随机扩增多态性DNA（RAPD）技术对取自大连、盘锦、东营、天津的4个地理种群的褶皱臂尾轮虫Brachionus plicatilis的遗传多样性进行了研究。在优化的RAPD反应条件下，对30个10bp的引物进行了PCR扩增，其中26条引物所得的扩增带带型清晰且重复性好，共检测出311个位点，片段长度为190～2000bp。根据4个种群之间的片段共享度和Nei的平均遗传距离得知，大连与天津种群的遗传距离最大，盘锦与天津种群之间的遗传距离最小。由UPGMA聚类可得：盘锦种群与天津种群聚在一起，然后与东营种群聚在一起，最后与大连种群聚在一起。

RAPD technique was applied to evaluate genetic diversity of four different rotifer（Brachionus plicatilis） populations which were collected from wild eggs in Dalian, Panjin, Dongying, Tianjin and cultured under the lab conditions. DNA of rotifers were extracted by frozen method and amplified DNA under the RAPD optimized conditions. In this study, amplification with 30 random primers produced 311 clear and reproducible stripes from 26 random primers. The length of amplified fragment was ranged from 190 to 2000 bp. According to a index of stripes sharing, Nei distance and clustering analysis among four populations the heredity distance between Dalian and Tianjin populations was the maximum. On the contrary, the heredity distance between Tianjin and Panjin populations was the minimum. Using UPGMA clustering analysis the Panjin and Tianjin populations were clustered into one group, followed by Dongying and Dalian populations.