摘要
研究了应用斑点酶联免疫吸附试验(Dot-Enzym e L inked Immunosorbent Assay,Dot-ELISA)技术检测副溶血弧菌Vibrio parahaem olyticus的方法。根据棋盘试验,确定副溶血弧菌免疫血清最佳工作浓度为1∶200,酶标抗体最佳工作浓度为1∶100,以出现明显清晰斑点者判定为阳性。用该方法检测时,副溶血弧菌呈阳性,哈维氏弧菌V.harveyi、嗜水气单胞菌Aerom onas hydrophila、河流弧菌Vf.luvialis biotype、溶藻弧菌V.alginolyticus、大肠杆菌Escherichia coli均呈阴性。斑点ELISA方法不仅具有快速、经济的特点,而且可用肉眼直接判定结果,适合在基层单位应用推广。
A Dot-Enzyme Linked Immunosorbent Assay (Dot-ELISA) was established for detection of Vibrio parahaemolyticus. The working concentration of serum samples was 1: 200 dilution and that of the enzyme - labeled goat anti -rabbit IgG was 1:100 dilution by chessboard assay. The samples with clear and obvious dots were judged as positive reaction. V. parahaemolyticus was positive in the Dot-ELISA, while V. harveyi, Aeromonas hydrophila , V. fluvialis biotype , V. alginolyticus and Escherichia coli were negative in the assay. Dot - ELISA was characterized by rapid reaction, economical feasibility, and observable results and widely adopted.
出处
《大连水产学院学报》
CSCD
北大核心
2006年第1期79-82,共4页
Journal of Dalian Fisheries University
基金
国家"863"高新技术研究发展计划项目(2002AA639470)