摘要
用高效阴离子交换色谱-脉冲安培检测法(HPAEC-PAD)测定了烤烟中的水溶性葡萄糖、果糖和蔗糖。采用水浸取及膜过滤法处理烤烟样品,以D ionex C arboPac PA-1阴离子交换柱为色谱柱,0.2m o l/L N aOH水溶液为淋洗液进行分离测定。葡萄糖、果糖和蔗糖的含量与其峰面积的线性关系良好,回收率均在97%以上。方法简便易行,灵敏度高,重现性良好,可以实现对烟草中单糖的快速分离和测定。
The quantitative determination of water-soluble sugars such as glucose, fructose and sucrose in flue-cured tobacco by high performance anion exchange chromatography coupled with pulsed amperometric detection (HPAEC-PAD) was developed. The method was used to determine flue-cured tobacco samples containing large amounts of carbohydrates after pretreatment by water-leaching and membrane filtration. The separation was performed on a Dionex CarboPac PA-1 anion exchange column with 0.2 mol/L NaOH solution as the eluent at a flow rate of 1.0 mL/min. The injection volume was 20μL. Good linearities were obtained for glucose, fructose and sucrose within the range of 0.5 - 100 mg/L (r^2 〉 0.997). The detection limits (S/N = 3) were 0.1 mg/L, 0.1 mg/L and 0.2 mg/L, and the average recoveries were 97.3%, 101.4% and 98.9% with good reproducibility for glucose, fructose and sucrose, respectively.
出处
《色谱》
CAS
CSCD
北大核心
2006年第2期201-204,共4页
Chinese Journal of Chromatography
基金
复旦大学分析测试中心与上海烟草(集团)公司合作项目
2003-2005年度复旦大学青年科学基金项目
关键词
高效阴离子交换色谱
脉冲安培检测法
葡萄糖
果糖
蔗糖
烤烟
high performance anion exchange chromatography (HPAEC)
pulsed amperometric detection (PAD)
glucose
fructose
sucrose
flue-cured tobacco