L-丝氨酸高产菌株的选育和摇瓶发酵条件优化

Breeding of L-Serine-Producing Strain and Optimization of Fermentation Conditions

采用B revibacterium flavmC-11A为出发菌株,经紫外线照射和亚硝基胍诱变处理,选育出一株L-丝氨酸高产菌株C32为目的突变株,使摇瓶产酸率由12.1 g.L-1增加到16.4 g.L-1,然后对其进行摇瓶发酵条件优化,使菌株C32的L-丝氨酸产率提高到30.1 g.L-1。

The strain Brevibacterium flavmC - 11A was mutated by ultraviolet radiation and NTG. As a result, a strain producing L - serine named C32 was obtained. The production of serine by flask - shaking fermentation increased from 12. 1g · L^-1 to 16.4g · L^-1 . Under optimum conditions, the production of serine by flask - shaking fermentation was up to 30. 1g · L^-1.