摘要
采用B revibacterium flavmC-11A为出发菌株,经紫外线照射和亚硝基胍诱变处理,选育出一株L-丝氨酸高产菌株C32为目的突变株,使摇瓶产酸率由12.1 g.L-1增加到16.4 g.L-1,然后对其进行摇瓶发酵条件优化,使菌株C32的L-丝氨酸产率提高到30.1 g.L-1。
The strain Brevibacterium flavmC - 11A was mutated by ultraviolet radiation and NTG. As a result, a strain producing L - serine named C32 was obtained. The production of serine by flask - shaking fermentation increased from 12. 1g · L^-1 to 16.4g · L^-1 . Under optimum conditions, the production of serine by flask - shaking fermentation was up to 30. 1g · L^-1.
出处
《氨基酸和生物资源》
CAS
2006年第1期46-48,共3页
Amino Acids & Biotic Resources
基金
国家十五攻关2004BA713B07-04
关键词
丝氨酸
选育
诱变
发酵
serine
mutation
breeding
fermentation