蔗糖和多效唑对试管生姜形成的影响

Effects of Sucrose and Paclobutrazol on Microrhizome Formation in Ginger(Zingiber officinale Rosc.)

用不同浓度的蔗糖、NAA、Ca3(PO4)2、多效唑(Pac)和光照条件对江西兴国九山生姜(ZingiberofficinaleRosc.cv.Jiushan)进行试管姜的诱导,成功诱导出试管姜。结果表明,诱导试管姜的最佳培养基为:MS+6-BA0.2mgL-1+NAA0.5mgL-1+Ca3(PO4)22.5mgL-1+Pac2.5mgL-1+蔗糖8%;适宜浓度的Pac、Ca3(PO4)2、蔗糖有利于试管姜诱导;NAA和6-BA对试管姜的诱导不起促进作用;延长光照时间有利于试管姜膨大。

Microrhizome of Zingiber officinale Rosc. cv. Jiushan were inducted from tube plantlets, which were derived from explant of stem tip, cultured on MS medium supplemented with 0.2 mg L^-1 BA and varying concentrations of combinations of sucrose, NAA, Ca3（PO4）2, Paclobutrazol （Pac）. The results showed that rhizome production was best in tube culture on MS medium supplemented with 0.2 mg L^-1 6-BA, 0.5 mg L^-1 NAA, 2.5 mg L^-1 Ca3 （PO4）2, 2.5 mg L^-1 Paclobutrazol and 8% sucrose. NAA and 6-BA had no promotion effects on rhizome induction. More illumination stimulatory rhizome growth of the ginger.