摘要
对鸡传染性喉气管炎病毒（ＩＬＴＶ）的鸡胚、鸡胚肾细胞等培养系统进行研究；对病毒包涵体、病毒形态进行观察；对病毒毒价进行测定。结果，病毒在鸡胚绒毛尿囊膜产生痘斑和坏死灶，鸡胚发育不良并致死；鸡胚肾细胞稳定地产生合胞体等细胞病变；绒毛尿囊膜切片经Ｈ－Ｅ梁色，可观察到紫红色嗜酸性核内包涵体充满细胞核；病毒细胞培养物粗提后用负染法电镜观察，绒毛尿囊膜用超薄切片法电镜观察，均可见到病毒颗粒，病毒直径约２４０～３００ｎｍ，核衣壳直径约１００～１４０ｎｍ，外有圆形或椭圆形囊膜；经微量培养系统测定，病毒毒价为１０６．１２ＴＣＩＤ５０／０．０５ｍＬ。
The cultural system of avian infectious laryngotracheitis virus (I LTV) including chichen embryo(CE) and chicken embryo kidney cell(CEK) has been researched, and the viral intranuclear inclusions and viral pattern have been observed, and the viral titer has been detected. The results demonstrated that I LTV can produce classic lesions in CE there are lot of pocks and necrotic fool in chorioallantoic membrane (CAM) and CE is dysplastic and dead, and CEK produces cytopathic effect such as syncytium. With H-E staining a lot of violet-red acidophilic intranuclear inclusions full of nuclei are found in CAM sections.By negative staining and electron microscope ultramicrotomy,the diameter of viron and nucleocapid are measured as about 240～300 nm and 100～140 nm respectively,and the viral envelope is observed to be circular or elliptical. Detected by microcultural system, the viral titer is 106.12TCID50/0. 05 mL.
关键词
传染性喉气管
病毒
培养系统
鸡病
avian infectious laryngotracheitis virus (I LTV)
cultural system
viral intranuclear inclusion
viral pattern
viral titer
