产志贺毒素大肠杆菌的分子生物学检测方法研究

STUDY ON BIOMOLECULAR TESTING METHOD FOR STEC

[目的]建立快速准确检测食品中产志贺毒素大肠杆菌的分子生物学方法。[方法]针对产志贺毒素大肠杆菌(STEC)的stx1、stx2、eaeA、hlyA4种毒力基因,设计了4对特异性引物。[结果]采用多重PCR(multiplexPCR)方法,得到614bp、779bp、450bp和300bp4条片断。[结论]实验结果表明,本文建立的mPCR方法较常规的大肠杆菌检测方法简便、快速、灵敏度高,灵敏度可达15cfu/mL。

A screening method is established by applying molecular biology, which is quick and precise to detect shiga toxinproducing Escherichia coli （STEC） in food. According to the four types of virulence genes： stxl, stx2, eaeA and hlyA, four pairs of specific primers have been designed. By using the method of multiplex PCR, the four segments of 614bp, 779bp, 450bp and 300bp are obtained. The result shows that the method is more simple, quick and precise than the usual method of detecting STEC with sensitivity up to 15efu/mL.