色谱指纹图谱相似度评价方法的规范化研究(二)

Study on the Standardization of Similarity Evaluation Method of Chromatographic Fingerprints (Part Ⅱ)

目的进行色谱指纹图谱相似度评价方法的规范化研究。方法采用高效液相色谱法和计算机拟合技术对特征成分(以北柴胡为例)的含量规律、指纹图谱的确立原则和相似度结果的统计学意义进行了考察。结果指纹图谱各指标成分的含量应符合正态分布规律;推荐采用成分含量信息与中位数构建参照指纹图谱;应对相似度结论的置信度进行考察。结论对色谱指纹图谱从样品收集、标准建立到结论检验等一系列环节从统计学意义上进行了阐述和规范。

Objective To study the standardization of similarity evaluation method of chromatographic fingerprints. Methods HPLC and computer simulation method were used to analyze the content variation pattern of the characteristic compounds, the construction method of standard fingerprints and the statistical meanings of similarity evaluation respectively. Results The content distribution of characteristic compounds should obey normal probability. It is recommended that content information and median algorism should be used to generate reference fingerprints. The confidence factor of similarity coefficient also should be tested. Conclusion The process of compound sampling, establishment of criteria and result test are described and standardized from statistical aspect.