SNP对生殖细胞凋亡作用的研究

Study on the effects of sodium nitroprusside on the germ cell apoptosis

目的探讨一氧化氮(NO)供体硝普钠(SNP)对大鼠睾丸生殖细胞凋亡的影响。方法采用末端脱氧核苷酸转移酶(TdT)介导的原位末端标记法(TUNEL),透射电镜和琼脂糖凝胶电泳检测生殖细胞凋亡的特征。结果TUNEL标记法检测表明,NO供体SNP可诱导生殖细胞的凋亡,并呈剂量时间依赖性,SNP组凋亡率明显高于对照组(P＜0.01)。透射电镜观察SNP处理15～20h后的生殖细胞,核染色质凝集,附着于核边缘呈新月形,核固缩、碎裂形成凋亡小体。琼脂糖凝胶电泳显示生殖细胞DNA片段呈现凋亡特征性的梯形区带。结论一氧化氮对生殖细胞的凋亡具有促使形成作用,这对不育症的研究有重要的价值。

Objective To study the effect of NO donor, sodium nitroprusside （SNP） on rat testis germ cell apoptosis. Methods TdT mediated dUTP nick, labeling（TUNEL） and transmission electromicroscopy （TEM） and agarose gel electrophoresis methods was adopted to detect the character of germ cell apoptosis. Results NO donor, sodium nitroprusside （SNP）, was found to induce apoptosis in germ cell, tachyzoites in a time and dose dependent manner by TUNEL detection. The apoptosis rate of SNP group was significantly higher than controls （P〈0.01）. Electron microscopy revealed that germ cell was observed chromatin condensation, marginate in crescents around the nuclear, nuclear pyknosis and formation of apoptotic body after 15 to 20 hour SNP-treated. Agarose gel electrophoresis revealed that germ cell DNA fragment exhibited characteristic ＂DNA ladder＂. Conclusion That NO may be involved in germ cell apoptosis was very important for infertile study.