肾衰合剂对急性肾衰竭大鼠的保护作用及其调节细胞因子分泌的实验研究

Experimental study on effects of shenshuai compound medicine on acute renal failure rats and secretion cell factors

目的:观察中药复方肾衰合剂对急性肾功能衰竭(ARF)大鼠的防治作用,揭示该方药的作用机制。方法:除正常对照组外,3组均予肌注甘油制备动物模型。分正常组、模型组、西药维拉帕米组、中药肾衰合剂(22.5 g.kg-1)4组灌胃,观察造模24 h大鼠肾脏超微结构、血一氧化碳(NO),内皮素(ET),肿瘤坏死因子(TNF-α)的变化。结果:肾衰合剂及维拉帕米均能显著改善ARF大鼠的肾功能,一定程度上保护初发期急性肾小管坏死(ATN)时肾小管上皮细胞等超微结构;同时肾衰合剂可显著降低ET、升高NO的含量,减少TNF-α的产生,其作用部分优于维拉帕米。结论:肾衰合剂通过调节血管活性因子及炎症因子的释放达到改善肾功能、减轻肾组织病变、防治ATN的作用。

Objective： To observe the effect of the experiential compound prescription Shenshuai compound medicine （SSCM） on acute renal failure （ARF） rats ,and expose its mechanism. Method： Male SD rots were allocated into four groups at random. Except normal group, the others were injected glycerin into the musculi to induce a model of ARF. At the same time, rots in normal and model groups were given a dose （10 mL·kg^-1） of normal saline; rots in the other two groups were given verapamil （Vp） 40 mg·kg^-1·d^-1 and SSCM 22.5 mg·kg^-1·d^-1 respectively by gastric garages. In this way they were killed at 24 h after injecting glycerin. Result： In contrast with model group, SSCM and Vp could ameliorate the renal function of acute tubular necrosis （ATN） rats markedly,in a way protect the renal inherent cell ultrastructure such as tubular epithelial cell etc at ATN early stage; especially SSCM could enhance NO contents in serum, and was reduce ET levels in plasma, evidently cut down TNF-α contents in serum, and was partly superior to Vp. Conclusion： It is indicated that SSCM can adjust the balance of contract and stretch blood vessal active substance and clear away initiate inflammatory medium, consequently alleviate renal pathological changes, prevent and treat ARF.