小麦族鹅观草属、披碱草属、猬草属和仲彬草属细胞质基因组PCR-RFLP分析

PCR-RFLP Analysis on Roegneria, Elymus, Hystrix and Kengyilia in Triticeae (Poaceae)

对鹅观草属、披碱草属、猬草属和仲彬草属23个物种和1份外类群共24份材料进行了细胞质基因组PCR-RFLP分析。3个叶绿体和3个线粒体通用引物扩增出的片段,用15种限制性内切酶对其进行酶切。在47种引物/酶组合中,获得329条DNA片段,其中304条具有多态性,占92.4%。结果表明鹅观草属、披碱草属、猬草属和仲彬草属材料存在属间和种间多态性,遗传相似系数较高。聚类分析显示仲彬草属单独聚为一类,鹅观草属R.grandis、R.aristiglumis、R.elytrigioides、R.alashanica、R.magnicaespes聚为一类,R.caucasica、R.ciliar-is、R.amurensis、R.japonensis聚在一起,5个披碱草属材料、3个猬草属材料和1个鹅观草属物种R.kamoji聚为一类。这些结果与前人对其进行的RAPD和RAMP分析的结果基本一致。仲彬草属作为属分类等级处理是恰当的,对于鹅观草属、披碱草属和猬草属的系统地位和一些物种的分类处理,有待进一步研究。

PCR-RFLP of Plasmon on 23 species of Roegneria, Elymus, Hystrix and Kengyilia and 1 outgroup （Triticum aestivum L. ） was carried out. The segments amplified with 3 cpDNA and 3 mtDNA universal primers were digested by 15 restriction endonucleases. Among 329 bands produced, 304 were polymorphic （92.4%）. PCR-RFLP results showed that polymorphisms existed between the four genera and between the species in Roegneria, Elymus, Hystrix and Kengyilia, and genetic similarity coefficient was high. Roegneria grandis, R. aristiglumis, R. elytrigioides, R. alashanica and R. magnicaespes were grouped, while R . caucasica , R . ciliaris , R . amurensis and R . japonensis clustered together. Species of Kengyilia clustered independently. Five species of Elymus, 3 of Hystrix and Roegneria kamoji grouped together. The results suggested Kengyilia was a valid genus. As more cytological and molecular data are available, the taxonomic status of Roegneria, Elymus and Hystrix will be recognized.