高效液相色谱法测定菠萝中多酚类化合物

Determination of the Phenolic Compounds in Pineapple by High Performance Liquid Chromatography

利用高效液相色谱法(HPLC)分析了多酚类物质在菠萝果皮、果肉、果心中的含量及分布情况。色谱条件：色谱柱为 Diamonsil C_(18)柱(250 mm×4．6 mm,5μm),流动相为乙腈和0．3%醋酸溶液,柱温为25℃,流速为 1．0mL/min,线性梯度洗脱,检测波长为280nm。在此色谱条件下,各组分均得到良好分离。经测定,菠萝果皮中含有4种酚类物质,为没食子酸、儿茶素、表儿茶素、阿魏酸,含量较高；果肉及果心中酚类物质为表儿茶素和阿魏酸,含量较低。

The content of phenolic compounds in pineapple peel, core and pulp were studied by high performance liquid chromatography（HPLC） on a C18 column（250 mm × 4.6 mm, 5μm）. The mobile phase was acetonitrile with 0.3 % acetic acid solution, The compositions were separated completely under the condition of： flow rate 1.0 mL/min, column temperature 25℃, 280 nm wavelength of detector. The determination results show that there were four hydrone phenolic compounds in pineapple peel, galic acid, catechin, epicatechin, and felinic acid; while in pineapple pulp and core,only little epicatechin and felinic acid were detected.