BDNF和神经干细胞联用对穹隆海马伞切割鼠基底前脑p75^(NGFR)阳性神经元数及其形态学的影响

Effect of neural stem cells and BDNF unite application on the number and morphology of p75NGFR positive neurons in basal forebrain in model rats with senile dementia

目的研究神经干细胞(NSC)和脑源性神经营养因子(BDNF)联合治疗对穹隆海马伞切割鼠基底前脑p75NGFR阳性神经元及其形态学的影响。方法切断SD大鼠左侧穹隆海马伞模拟AD大鼠模型,利用无血清培养技术获得新生SD鼠的海马NSC,基底前脑注射NSC,同时侧脑室注射BDNF,4周后行免疫组化结合图象分析技术观察各组大鼠基底前脑p75NGFR阳性神经元及其形态学变化。结果损伤组大鼠p75NGFR阳性神经元数在内侧隔核(MS)和斜角带(VDB)较正常组明显下降(P<0.01);移植组细胞数较损伤组有改善(P<0.05);与正常组相比较,联合组免疫阳性神经元数无显著下降(P>0.05)。形态学参数测试结果显示,p75NGFR阳性神经元的面积、周长在4组中的改变类似p75NGFR阳性神经元数。结论NSC和BDNF联用较单独使用BDNF或NSC更好地增加p75NGFR阳性神经元数及其形态学参数。

Objective To study the effect of neural stem cells（NSCs） and brain derived neurotrophic factor（BDNF） unite application on the number and morphology of p75NGFR positive neurons in basal forebrain in rats with Alzheimer＇s disease. Methods NSCs were obtained from hippocampus by using the serum-free culturing. NSCs were transplanted into the transected basal forebrain , then BDNF was injected into lateral ventricle. After 4 weeks, the number and morphology of p75NGFR positive neurons was analyzed by using immunohistochemical method combined with technique of micromeasure and image analysis. Results The number of p75NGFR positive neurons for injured groups in medial septum （MS） and vertical diagonal branch（VDB） was decreased compared with normal control groups （P〈 0.01）; The number for NSCs groups had more significant improvement than that of the injured groups （P〈0.05）, compared with normal control groups, there was a significant diference （P〈0.05）; But no significant difference between the combination groups and normal control groups （P〈0.05）. For the morphology of p75NGFR positive neurons in four groups is assemble to the number of p75NGFR positive neurons. Conclusions Combination treatment of NSCs and BDNF is more effective in improving the number and morphology of p75NGFR positive neurons in model rats with Alzheimer＇s disease thantrea ted with BDNF or NSCs alone.