云芝菌丝球在流化床反应器中产漆酶的研究

Laccase production by mycelial pellets of Coriolus versicolor in fluidized-bed bioreactor

为了提高云芝菌发酵生产漆酶的效率,提出了一种利用自絮凝菌丝球在流化床生物反应器中重复分批发酵产漆酶的新方法.采用流化床生物反应器中所得漆酶对含靛蓝的染整废水进行脱色研究.在优化后的产酶条件下,考察苯酚的添加浓度对漆酶活力的影响,并通过在培养基中添加苯酚诱导物进行漆酶多批次培养.研究结果表明,重复8批产酶的平均漆酶活力高达694.733单位/mL.此方法所得漆酶对染料靛蓝具有明显的脱色降解作用,当酶用量为2.78单位/mL废水,反应40min,脱色率达96.5%.该方法采用的流化床生物反应器性能稳定、菌丝球可重复使用,该方法有利于漆酶的高效、规模化生产.

To improve the laccase fermentation productivity of coriolus vericolor, a novel method for laccase fermentation production was proposed in a fluidized-bed bioreactor with the mycelial pellets undergoing repeated batch processing. Decolorization experiments on laccase produced in a fluidized-bed bioreactor were used to study dyeing wastewater containing Indigo blue. Under the optimum laccase production conditions, the effects of the added phenol concentration on laccase activity were investigated, and laccase was cultured by adding inducer phenol in culture medium under repeated batch processing. Results show that the average laccase activity of 8 batches is 694. 733 mL^-1. Laccase produced by the method decolorizes indigo blue markedly. When the laccase dosage in the dyeing wastewater is 2.78 mL^-1 , the decolorization rate reaches 96.5% in 40 min. The performance of the fluidized-bed bioreactor is stable, and the mycelial pellets can be used repeatedly to effectively yield laccase in large scale by the method.