卫星搭载胡萝卜组织培养研究

Tissue Culture of Daucas carta L.Treated by Space Flight

以经卫星搭载的胡萝卜种子发育而来的嫩叶为外植体,诱导愈伤组织,探讨诱导后胡萝卜愈伤组织对外源激素的反应.实验结果表明,诱导松散型愈伤组织的最佳激素配比是1.0 mg/L 2,4-D+0.5 mg/L 6-BA.此愈伤组织移入含有0.1 mg/L的TDZ培养基中能产生大量的芽,但培养20 d左右芽膨大透明成绿色,之后将芽移入含有不同种类不同浓度的生根培养基中都能生根且易于移栽.本实验为进一步筛选胡萝卜抗胁迫变异系奠定了基础.

The seeds of Daucas carta L. were treated by space flight, and young tender leaves of the germinated plants were used as explants for callus induction,testing the effects of different exogenous hormones on the tissue culture. The results indicated that callus were efficiently induced on Murashige and Skoog （MS） medium supplemented with 1.0 mg L^-1 2,4 - dichlorophenoxyacetic acid （2,4 - D） and 0.5 mg L^-1 6 - benzylaminopurine （6 - BA）. A high frequency of regenerated shoots was obtained on MS medium with 0.1 mg L^-1 Thidiazuron （TDZ） but vitrifiated gradually after 20 days of culture. After 20 days regenerated shoots were transferred to rooting medium with or without different various cytokinin. All of them regenerated roots and could be transplanted. The experiment could be used for the selection of stress tolerant flax lines on Daucas carta L, and also lay a foundation for the future.