摘要
用荧光光谱法和紫外-可见光谱法研究了在模拟人体生理条件下,氯氮平和牛血清蛋白(BSA)结合反应的特征,发现氯氮平对BSA有较强的荧光猝灭作用,且氯氮平的紫外吸收光谱和BSA的荧光光谱有一定程度的重叠,由此可得出其作用距离和结合过程的基本热力学参数。
The binding characteristics of clozapine（CP） and bovine serum albumin（BSA） in aqueous solution were studies with fluorescence and absorption spectrometric methods. The results showed that CP had a powerful ability to quench the BSA fluorescence via nonradiative energy transfer mechanism. The fluorescence quenching data were analyzed according to Scatchard equation and double-reciprocal equation. The reaction distance and the thermodynamic parameters were obtained.
出处
《分析测试学报》
CAS
CSCD
北大核心
2006年第2期25-28,共4页
Journal of Instrumental Analysis
基金
国家自然科学基金资助项目(20275028)
关键词
氯氮平
牛血清白蛋白
荧光光谱法
吸收光谱法
Clozapine
Bovine serum albumin
Fluorescence spectrum
Absorption specturm
