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手性氨基酸的毛细管电泳拆分研究 被引量：8

Chiral Separation of Amino Acids by Capillary Electrophoresis
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摘要 以咔唑-9-乙基氯甲酸酯(CEOC)作柱前荧光衍生试剂,在毛细管区带电泳和胶束电动色谱条件下,以不同配比的β-环糊精(-βCD)和脱氧胆酸钠(SDC)作手性选择剂,在两种不同分离模式下,对氨基酸衍生物进行了手性分离,同时对分离过程中的相关参数进行了优化,分别实现了8种和10种氨基酸的快速手性分离。 Simple and rapid methods for the chiral separation of amino acid enantiomers using 9-（2- carbazole） ethyl chloroformate （ EEOC ） as derivation reagent by capillary zone electrophoresis （CZE） and micellar electrokinetic chromatography（MEKC） with diode array detector at 235 nm have been developed. Based on cooperative effect of binary chiral selectors,β-eyclodextrin（β-CD） and sodium deoxycholate（SDC） were investigated as chiral selectors, and the optimum molar ratio of β-CD to SDC for the chiral separation of each of amino acid enantiomers was obtained. Under CZE and MEKC separation modes, eight and ten pairs of amino acid enantiomers were separated with a good baseline resolution respectively. At the same time, the effect of some important parameters on the chiral separation was also investigated.
作者 程燕 白敏 石运伟 明永飞 尤进茂
机构地区 曲阜师范大学化学科学学院
出处 《分析测试学报》 CAS CSCD 北大核心 2006年第2期52-55,59,共5页 Journal of Instrumental Analysis
基金 国家自然科学基金资助项目(20075016)
关键词 手性分离 毛细管电泳 氨基酸 Β-环糊精 脱氧胆酸钠 Chiral separation Capillary electrophoresis Amino acids β-Cyclodextrin Sodium deoxycholate
分类号 O657.8 [理学—分析化学] Q517 [生物学—生物化学]
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参考文献14

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二级参考文献26

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二级引证文献58

分析测试学报
2006年 第2期

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