胚胎期乙醇暴露对大鼠听性脑干反应的影响

Effects of prenatal alcohol exposure on auditory brainstem response in rats

目的:建立胚胎期乙醇暴露的大鼠动物模型,测试不同剂量乙醇暴露下ABR,探讨乙醇胚胎毒性对大鼠听觉神经系统的影响。方法:母鼠交配后第8.5天经灌胃途径按高、低剂量组每天分别给予50.0%分析纯乙醇2.0 g/kg、1.0 g/kg,连续6 d。仔鼠40 d龄后测试ABR,选择相同天龄仔鼠作为正常对照组。结果:高、低剂量的胚胎期乙醇暴露对ABR有影响,表现为I波测出率降低(分别为14.0%、30.0%)、各波潜伏期延长(P<0.05)和波幅降低。结论:乙醇胚胎毒性对大鼠听觉神经系统有明显影响,听觉神经冲动传导减慢。

Objective： To study the effects of prenatal alcohol exposure, with an animal model of auditory brainstem response recorded in the conditions of various alcohol dosages administrated during embryonic period,on auditory nervous system in rats. Method：Normal female rats were divided into 2 groups. From the days 8. 5 after mating, pregnant rats were fed 50% analytical reagent alcohol via intragastric administration with 2.0 and 1.0 g/ （kg·d） respectively in consecutive 6 days. On the days 40 after birth, ABR was recorded in young rats under anesthesia. Normal young rats with parallel age were chosen as the normal group. Result：Prenatal alcohol exposure had effects on ABR, manifesting the incidence rate of I wave lowing（ 14 % ,30 % ）,latency delaying（ P 〈0.05） and amplitude reducing. Conclusion： Prenatal alcohol exposure had conspicuous effects on auditory nervous system in rats, by step down nerve impulse conduction.