高效液相色谱-光电二极管阵列检测器法测定齐墩果酸原料及片剂中齐墩果酸的含量

Determination of Oleanolic Acid in Materialand Oleanolic Acid Troches by HPLC-PAD

目的建立齐墩果酸原料及片剂中齐墩果酸含量的测定及齐墩果酸紫外光谱鉴定方法。方法运用高效液相色谱-光电二极管阵列检测器法。色谱柱为Nova—Pak C18，流动相为乙睛-水（65：35），流速0．8mL／min，检测波长210nm，柱温25℃。结果齐墩果酸在3．48—17．4μg范围内线性关系良好（r=0．9999），检出限为0．3mg／L（3σ），平均回收率为99.5％。结论该方法简便，准确，线性范围宽，重现性好，适于对含有该成分的药物进行质量评价。

Objective A determination method of oleanolic acid in material and oleanolic acid troches by reversed - phase high performance liquid chromatograph was established and the purity of oleanolic acid was detected by means of Photodiode Array Detector. Methods The chromatographic column, Nova-Pak C18（3.9 mm × 150 mm, 5 μm）, ethanenitrile-water （65：35, V/ V） mobile phase with 0.8 ml/min flow rate, the detected wavelength （210 nm）, and the column temperature （25 ℃） were adopted. Results The calibration curve of oleanolic acid was linear under the content of 3.48 - 17.40 μg, the correlation coefficient was over 0,999 9, the detected limit was 0.3 mg/L （3σ）. The average recovery of oleanolic acid was 99.5%. Conclusion The method is simple,accurate and has wider linear range. The method can be adapted to evaluate the quality of medicinal plants which contain oleanolic acid.