三七总皂甙对培养的猪主动脉内皮细胞释放一氧化氮的影响

The Effect of Total Panax Notoginseng Saponin on Releasing Nitric Oxide from Cultured Porcine Aorta EndothelialCells

本文观察了中药田七的主要药用成分三七总皂甙对培养的猪主动脉内皮细胞分泌一氧化氮以及形态学的影响。结果表明加有三七总皂甙条件培养液的猪主动脉内皮细胞生长良好，仍融合成片。而对照组的猪主动脉内皮细胞出现明显收缩，胞浆中有大量小空泡。猪主动脉内皮细胞在三七总皂甙的条件培养液中培养２４小时后，条件培养液中一氧化氮含量由对照组的５５ｎｍｏｌ·Ｌ－１上升到平３９０ｎｍｏｌ·Ｌ－１（Ｐ＜０．００１）。从培养的猪主动脉内皮细胞在加入三七总皂甙条件培养液后不同时间检测其条件培养液中的一氧化氮含量也表明三七总皂甙能在短时间内刺激内皮细胞分泌一氧化氮。结果提示三七总皂甙能明显增加培养中的猪主动脉内皮细胞分泌一氧化氮，对培养的内皮细胞的生长有维持和保护作用。

Aim Total Panax Notoginsen saponin(tPNS) is a traditional herb medicine of Chinese. In this study, we have investigated the release of nitric oxide (NO) from cultured porcine aorta endothelial cells (PAEC) affected by the tPNS for understanding the pharmacological mechanism of tPNS.Methods PAEC were cultured with M199 and compared the morphological appearence of tPNS group with the control under the phase contrast microscope after PAEC exposed to conditional medium (CM) for 24 hours. NO amounts in CM were determinated by the methods of Termin and Green.Results PAEC in tPNS groups were grew normally. The cells of control group were constricted and separated from each other, there were a lot of small vesicles in the cytoplasma. The amounts of NO in CM of tPNS groups were ranged from 350±59 to 410±90nmol·L-1, and compared with the control, the P<0.001. This enhance was ceased by pluinq EDTA in CM. The time course expressed that the amount of NO was raised quickly by pluing tPNS and was maintaining a high level. This effect could stop by adding the EDTA in CM too.Conclusions The results of enhancing the release of NO from PAEC and maintaining the growth of PAEC by tPNS were suggested that the tPNS can playa significant role in some diseases such as atherosclerosis.