河蚌多糖提取工艺的优化

Optimum Extraction Process of Mussel Polysaccharide

目的对河蚌多糖的提取工艺进行优化,从而提高多糖的含量。方法采用正交设计的方法,以硫酸蒽酮法测定总糖含量为评价指标,从提取温度、NaOH浓度、提取次数等因素入手,进行正交优化实验。结果提取温度、NaOH浓度为有极显著意义的因素,提取次数对多糖的含量影响不大,综合考虑,最佳提取工艺为A1B2C1,即在4℃时,NaOH浓度为5%,提取1次。结论优选得到的工艺经济,简单、稳定、可行。

Objectlve Optimize the extraction process of Mussel Polysaccharide（MPa） , and then gain higher content of polysaecharide. Methods Involved in the three factors, temperature and times of extraction, concentration of NaOH solutions, do the experiment by orthogonal design uniform design and the method of anthrone. Results The result show that the most important factors are temperature of extraction and concentration of NaOH solutions. The optimum extraction conditions were A1B2C1, 4℃ ,5% NaOH solutions and extracting only once . Conclusion This optimized process is economical, simple, stable and efficient.