摘要
本研究以氢过氧化枯烯作为脂质过氧化反应的引发剂,作用于培养的牛主动脉内皮细胞,制备内皮细胞条件培养液,测定其对Swiss3T3细胞DNA合成的影响;并以抗血小板源性生长因子(platelet-derivedgrowthfactor,PDGF)抗体中和实验和Northernblot分析测定了脂质过氧化作用对内皮细胞PDGF产生的影响。结果表明:氢过氧化枯烯使内皮细胞内脂过氧化物蓄积;氢过氧化枯烯组内皮细胞条件培养液作用的Swiss3T3细胞[3H]-TdR的掺入量明显高于正常对照组;以抗PDGF-AA、抗PDGF-BB中和抗体处理后,氢过氧化枯烯作用组的内皮细胞条件培养液对Swiss3T3细胞的增殖促进活性未见减弱;并且以氢过氧化枯烯作用后内皮细胞PDGF-B链mRNA的水平比正常组有所减低。提示,由氢过氧化枯烯引发的脂质过氧化反应,使内皮细胞源性生长因子的分泌增加;并且可能为PDGF以外的分子物质。
Aim The purpose of this paper is to investigate whether the lipid peroxidation of endothelial cells had any effect on the production of growth factors by them.Methods The bovine aortic endothelial cells were isolated and cultured using an enzyme-dispersal method.and the media conditioned by endothelial cells with lipid peroxidation induced by cumene hydroperoxide (LPECCM) were collected. The mitogenic activity of LPECCM for Swiss 3T3 cells was determined by incorporation of[3H]-thymidine into DNA of the cells.The effect of lipid peroxidation of endothelial cells on their synthesis and secretion of PDGF was performed with a neutralization assay using anti-PDGF antibodles and Northern blot analysis. Results Cumene hydroperoxide induced storage of lipid peroxide in cultured endothelial cells, and there was an obvious increase in the incorporation of[3H] thymidine into DNA in wiss 3T3 cells treated with LPECCM compared with the control.However.the mitogenic activity of LPECCM for Swiss 3T3 cells were not inhibited by anti-PDGF-AA and anti-PDGFBB antibodies. Conversely, a weaker PDGF-B chain mRNA expression in endothelial cells was observed after exposure to cumene hydroperoxide.Conclusions It seems reasonable to believe that lipid peroxidation of endothelial cells induced by cumene hydroperoxide leads to an increased synthesis and secretion of growth factors other than PDGF in cultured bovine aortic endothelial cells.
出处
《中国动脉硬化杂志》
CAS
CSCD
1996年第1期24-27,共4页
Chinese Journal of Arteriosclerosis
关键词
内皮细胞
生长因子
动脉粥样硬化
过氧化脂质
Lipid peroxidation
Endothelialcell-derived growth factor
Atherosclerosis