API0134对氧自由基诱导内皮细胞表达c－sis　mRNA和血小板源性生长因子B链的影响

Effects of API0134 on Expression of csis mRNA and Platelet-derived Growth Factor B Chain Protein in Endothelial Cells Induced by Oxygen Free Radical

本文应用免疫组织化学和原位杂交方法，观察中药穿心莲成分ＡＰＩ０１３４对氧自由基诱导猪主动脉内皮细胞表达癌基因ｃ-ｓｉｓｍＲＮＡ和血小板源性生长因子Ｂ链的影响。结果是，氧自由基能诱导内皮细胞ｃｓｉｓｍＲＮＡ的转录和促血小板源性生长因子Ｂ链表达。ＡＰＩ０１３４能显著抑制氧自由基的上述诱导作用，抑制效应与药物浓度呈正相关。因此提示，ＡＰＩ０１３４的这一作用可能是其抗动脉粥样硬化的机制之一。

Aim The effects of API0134 on expression of c-sis mRNA and platelet-derived growth factor B chain protein(PDGF-B)in porcine aortic endothelial cells (EC)induced by oxygen free radical (OFR) were studied in this article.Methods Immunohistochemistry,in situ hybridization and computer image analysis methods were used.Results The results showed,that OFR induces csis mRNA transcription and stimulates PDGF-B expression in EC;The inducible effects of OFR can be inhibited by API0134,the inhibitory effects are dose dependent.Conclusion These data suggest that API0134 may play a role in anti-atherosclerosis.