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氧化型低密度脂蛋白介导单核细胞与血管内皮细胞粘附的单细胞定量研究 被引量:1

Monocellular Quantitative Study on the Adhesion of Monocyte-Vascular Endothelial Cell Mediated by Oxidized Low Density Lipoprotein
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摘要 利用细胞微管吸吮技术对单核细胞与血管内皮细胞间的粘附力行单细胞定量测量,以两种细胞间的临界分离应力Sc来表示细胞的粘附性。观察氧化型低密度脂蛋白对单核细胞与培养人脐静脉内皮细胞粘附的影响。结果显示:氧化型低密度脂蛋白可使内皮细胞对正常单核细胞的粘附性明显增大,其发生较缓慢,4h达峰值并维持至24h以后。经氧化型低密度脂蛋白处理的内皮细胞对中性粒细胞的粘附性无明显提高。单核细胞和中性粒细胞经氧化型低密度脂蛋白处理后,对内皮细胞的粘附性均有迅速提高,1h达峰值,24h内逐渐减弱。实验结果提示氧化型低密度脂蛋白可选择性地提高单核细胞与内皮细胞相互粘附性,这种选择性的促粘附作用进一步证明它在动脉粥样硬化发生、发展中起重要作用。 The ability of human monocyte (MC)to interact with human umbilical vein endothelial cell (VEC) midiated by oxidized low density lipoprotein (OLDL) was determinated by the use of a micromanipulation technique. The ability of polymorphonuclear neutrophil (PMN) was observed at same condition to show the speciality of OLDL’s effect.The results showed that treatment of VEC with OLDL caused a significant increase in critical separation stress (Sc) MC-VEC.contrast, of PMN-VEC was not increased.Treatment of MC and PMN with OLDL caused a repid increase in Sc of MC-VEC and PMN.VEC,the Sc was maximal within 1 hour of exposure to OLDL and decreased in 24 hours.The results suggest that OLDL can enhance MC-VEC interation specially, this effect indicate that OLDL is important factor for the binding of MC to VEC and the entry of MC into the vessel wall in atherosclerotic lesion.
作者 周向东 黄勇
出处 《中国动脉硬化杂志》 CAS CSCD 1996年第1期32-34,共3页 Chinese Journal of Arteriosclerosis
关键词 动脉粥样硬化 单核细胞 病理 低密度脂蛋白 Atherosclerosis Lipoprotein lowdensity Cell adhesion Monocyte
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