壳聚糖-明胶-磷酸三钙组织工程支架用于兔颅骨缺损的修复

Chitosan-gelatin/β-tricalcium phosphate composite as bone tissue engineering scaffolds

目的探索壳聚糖-明胶-磷酸三钙作为骨组织工程支架用于骨缺损修复的可行性。方法采用二次冻干技术制备孔径200-400μmCS-Gel/TCP三维立体支架材料;新西兰兔12只随机分为两组,将兔骨髓基质细胞(MSC)进行体外培养、扩增、诱导为骨髓基质成骨细胞(MSO),并制备直径为15mm的颅骨全层圆形骨缺损。实验组与颅骨缺损处植入等大的MSO-支架复合体,对照组植入单纯支架,分别4、8周取材,组织切片与X线观察骨形成情况。结果MSO-支架复合体植入骨缺损区后,具有良好的骨修复效果,明显优于对照组。结论壳聚糖-明胶-磷酸三钙海绵状复合体是一种具有潜在临床应用价值的骨组织工程的支架材料组织工程。

Objective To explore the feasibility of Chitosan （CS）-Gelatin/β-tricalcium phosphate （TCP） composite as bone tissue engineering scaffolds.Methods The CS-Gel/β-TCP sponges were prepared by freezing and lyophilizing,which exhibit a three-dimensional porous structure with 200-400μm pores.12 New Zealand White rabbits were randomly divided into two groups.The marrow stromal osteogenic cells（MSC） were collected,cultured,multiplied, and promoted into marrow stromal osteogenic cells（MSO）.And the round defects of diameter 15 mm were created in the parietal bone, and the same size cell-sponge constructs were used to cover these defects, but only sponges were used as controls.The rabbits were killed after 4 or 8 weeks.Histology and X-rays were used to evaluate healing of the defects. Results Most area of the MSCs-scaffold complex transplanted defects was filled with newly formed bone.While in simplicial sponge scaffolds implanted defects bone formation was only noticed at the edge of host bone.Conclusion CS-Gel/β-TCP sponge complex is one latent importance bone tissue engineering scaffole.