睾酮对慢性心力衰竭雄性大鼠左心功能的影响

Effect of physiological dose testosterone on left ventricular function of male rats with chronic heart failure

目的:观察生理剂量睾酮(Testosterone,T)替代治疗对心力衰竭雄鼠左心功能的影响。方法:健康雄性SD大鼠120只,随机分为假术组(sham组,n=15),模型组(n=105)。模型组行冠脉结扎术,术后6周模型组存活44只,随机分为治疗组(CHF+TU,n=22)、心衰对照组(CHF,n=22),治疗组给予十一酸睾酮(testosterone undecanonate TU)5 mg/kg/次,1次/2周深部肌肉注射。心衰对照组给等容积的灭菌花生油。给药前、睾酮治疗12周后分别行超声学检查以评价心功能。结果:心力衰竭大鼠T水平与Sham组比较明显降低(179.3±89.81 ng/dl vs.475.28±161.37 ng/dl,P<0.05),TU治疗12周后CHF+TU组大鼠左室射血分数(EF)与CHF组比较明显改善(36.1±9.3%vs.33.2±7.5%,P<0.05)。结论:雄性慢性心力衰竭大鼠T水平明显降低,生理剂量的T替代治疗可以改善心衰大鼠左心功能。

Objective： To investigate the effects of physiological dose testosterone undecanonate（TU） on the left ventricular function of male rats with heart failure after myocardial infarction. Methods ： 120 SD male rats were randomly assigned to ligation of coronary artery （ n = 105） or sham operation （sham, n = 15）. 6 weeks later , 44 survived model rats were randomly divided into 2 groups： CHF ＋ TU group （ n = 22） treated with TU 5 mg/kg per 2 weeks intramuscular injection; CHF group（ n = 22） treated with placebo. Serum testosterone levels were determined after treatment for 12 weeks. The left ventricular functions were examined by echocardiography. Results ： In CHF male rats EF of left ventficular compared with the Sham group were greatly decreased（33.2 ± 7.5% vs.69.1 ± 6.7%, P 〈 0.05） companying with the decrease of serum testosterone level（ 179.3 ± 89.81 ng/dl vs. 475.28 ± 161.37 ng/dl, P 〈 0. 05）. After physiological dose TU administration the EF of the CHF ＋ TU group rats improved significantly compared with its of the sham group rats （36.1 ± 9.3 % vs. 33.2 ± 7.5 %, P 〈 0.05）.Conclusion ： The serum level testosterone of male rats with heart failure after myocardial infarction was decreased significantly. Physiological dose testosterone treatment can improve left ventricular systolic function.