从性病患者中分离培养沙眼衣原体

Isolation of Chlamydia trachomatis from STD Patients in Fujian

经多聚酶链反应（ＰＣＲ）及荧光标记的沙眼衣原体单克隆抗体（ＭｃＡｂ）染色初筛试验显示沙眼衣原休（Ｃｈｌａｍｙｄｉａｔｒａｃｈｏｍａｔｉｓ，Ｃｔ）阳性的１４份性病患者泌尿生殖道标本，用ＤＥＡＥ－葡聚糖及放线菌酮（Ｃｙｌｏｈｅｘｉｍｉｄｅ）处理的ＭｃＣｏｙ细胞进行沙眼衣原体分离培养，通过荧光标记的抗单克隆抗体（ＭｃＡｂ）染色及ＰＣＲ试验证实。其中９份标本阳性。其余阴性。从而首次在福建省从泌尿生殖道分离出沙眼衣原体。

Chlamydia trachomatis(Ct)was isolated with DEAE-dextran and cycloheximide treated McCoy cell from 14 sexual transmission disease(STD)patients'specimens which had been shown Ct positive by two kinds of testing befor the pathogenic agent was isolated.The isolations were identified by FITC-conjugated monoclonal antibody(McAb)against Ct and PCR assay.Of all the 14 speciments tested,9 were positive,Thus it was evidently proved for the first time that urogenital infection of CT also exists in Fujian.