Differences in oxidative stress dependence between gastric adenocarcinoma subtypes

瞄准：在与他们的病理学的标准和组织学的子类型的关系在 pre 肿瘤、肿瘤的胃粘膜调查氧化应力的程度。方法：从 98 个病人的 104 胃的腺癌的一个总数(88 渗透性并且 16 个 intraepithelial 肿瘤)为 i NOS 的表示和包含包含 DNA ，，的蛋白质和 8-hydroxy-2'-deoxyguanosine ( 8-OH-dG )的 nitrotyrosine ( NTYR )的出现组织化学地是估计的免疫到蛋白质和 DNA 的没有生产和损坏的标记。结果：为 i NOS， NTYR 和 8-OH-dG 染色的肿瘤房间分别地在 41% 渗透性的癌， 62% 和 50% 被检测。三个标记在 intraepithelial 癌第一次被显示出。i NOS 的表示是显著地在与弥漫的癌(DC ) 相比的管状的癌(TC ) 更经常(54% 对 18% ；P = 0.008 ) 或在多形的癌(PolyC )(54% 对 21% ；P = 0.04 ) 。染色的 NTYR 更经常显然在 PolyC 比那在 TC 被发现(72% 对 30% ；P = 0.03 ) 。向当远转移(下午) 是在场的时，染色的 i NOS 的更高的率有一个趋势。在渗透性的 TC，氧化压力标记的存在显著地没与组织学的等级，发炎的密度，渗入(磅) 的深度，淋巴节点传播(pN ) 和病理学的阶段(pTNM ) 被相关。结论：iNOS 氧化的小径可以在 TC 起一个重要作用，但是中等在 PolyC 和 DC。DNA 氧化和蛋白质硝代发生在三种子类型。基于 NTYR 层次， TC 和 PolyC 的有效差量作为二种不同子类型出现。

AIM： To investigate the extent of oxidative stress in preneoplastic and neoplastic gastric mucosa in relation to their pathological criteria and histological subtypes. METHODS： A total of 104 gastric adenocarcinomas from 98 patients （88 infiltrative and 16 intraepithelial tumors） were assessed immunohistochemically for expression of iNOS and occurrence of nitrotyrosine （NTYR）-containing proteins and 8-hydroxy-2＇-deoxyguanosine （8-OH- dG）-containing DNA, as markers of NO production and damages to protein and DNA. RESULTS： Tumor cells staining for iNOS, NTYR and 8-OH-dG were detected in 41%, 62% and 50% of infiltrative carcinoma, respectively. The three markers were shown for the first time in intraepithelial carcinoma. The expression of iNOS was significantly more frequent in tubular carcinoma （TC） compared to diffuse carcinoma （DC） （54% vs 18%; P=0.008） or in polymorphous carcinoma （PolyC） （54% vs 21% ; P=0.04）. NTYR staining was obviously more often found in TC than that in PolyC （72% vs 30% ; P=0.03）. There was a tendency towards a higher rate of iNOS staining when distant metastasis （pM） was present. In infiltrative TC, the presence of oxidative stress markers was not significantly correlated with histological grade, density of inflammation, the depth of infiltration （pT）, lymph nodes dissemination （pN） and pathological stages （pTNM）. CONCLUSION： The iNOS-oxidative pathway may play an important role in TC, but moderately in PolyC and DC. DNA oxidation and protein nitration occur in the three subtypes. Based on the significant differences of NTYR levels, TC and PolyC appear as two distinct subtypes.