Eubacterium limosum ameliorates experimental colitis and metabolite of microbe attenuates colonic inflammatory action with increase of mucosal integrity

瞄准：为了检验真细菌类高级轿车的效果，加(E。高级轿车和) 在结肠的上皮的房间线在试管内上，并且到评估 E 的效果。试验性的大肠炎上的高级轿车和。方法：E。高级轿车和被接种厌氧的联盟者和它的代谢物被获得。E 的生长 stimulatory 效果。T84 房间上的高级轿车和代谢物被 IL-6 生产被 SUDH 活动，和反煽动性的效果评估。在象受体 4 一样的使用费(TLR4 ) 的 mRNA 的变化被实时 PCR 评估。大肠炎被用 2.0% 葡聚糖硫酸钠喂 BALB/C 老鼠导致。这些老鼠收到了任何一个 5% lyophilized E。高级轿车和(n = 7 ) 或控制节食(n = 7 ) 。在大肠炎正式就职，临床、组织学的分数，冒号长度，和盲肠的有机酸层次以后的七天是坚定的。结果：E。高级轿车和在 0.25， 1.0， 0.025 和 0.07 mmol/L 生产了丁酸盐，醋酸盐，丙酸盐，和 lactate，分别地在里面中等。在这集中，每酸没有生长在 T84 细胞上刺激活动；然而，当这些酸在上述层次一起被混合时，它比控制显示出显著地高的活动。除了 lactate，这些酸显著地在就 0.1 mmol/L 稀释了 IL-6 生产。在 TNF-alpha 刺激下面，另外，丁酸盐稀释了 TLR4 mRNA 的生产。有 E 的处理。高级轿车和显著地随盲肠的丁酸盐层次的增加稀释了大肠炎的临床、组织学的分数，与控制组相比。结论：E。高级轿车和能改善试验性的结肠的发炎。部分地， E 的代谢物。高级轿车和，丁酸盐，增加粘膜正直和表演经由 TLR4 的粘膜防卫系统的反煽动性的行动调整。

AIM： To examine the effect of Eubacteriuro Iiroosuro （E.Iiroosuro） on colonic epithelial cell line in vitro, and to evaluate the effect of E.limosum on experimental colitis. METHODS： E.Iimosum was inoculated anaerobically and its metabolites were obtained. The growth stimulatory effect of the E.limosum metabolites on T84 cells was evaluated by SUDH activity, and the anti-inflammatory effect by IL-6 production. The change in mRNA of toll like receptor 4 （TLR4） was evaluated by real time PCR. Colitis was induced by feeding BALB/C mice with 2.0% dextran sodium sulfate. These mice received either 5% lyophilized E.lirnosum （n=7） or control diet （n=7）. Seven days after colitis induction, clinical and histological scores, colon length, and cecal organic acid levels were determined. RESULTS： The E.Iimosum produced butyrate, acetate, propionate, and lactate at 0.25, 1.0, 0.025 and 0.07 retool/L, respectively in medium. At this concentration, each acid had no growth stimulating activity on T84 cells; however, when these acids were mixed together at the above levels, it showed significantly high activity than control. Except for lactate, these acids significantly attenuated IL-6 production at just 0.1 mmol/L. In addition, under TNF-α stimulation, butyrate attenuated the production of TLR4 mRNA. The treatment with E.limosum significantly attenuated clinical and histological scores of colitis with an increase of cecal butyrate levels, compared with the control group. CONCLUSION： E.limosum can ameliorate experimental colonic inflammation. In part, the metabolite of E.lirnosurn, butyrate, increases mucosal integrity and shows anti-inflammatory action modulation of mucosal defense system via TLR4.