重寄生链霉菌F46与生防放线菌SC11融合菌株的筛选

Identification of recombinants of hyperparasitic Streptomyces F46 and biocontrol actinomycetes SC11

利用重寄生链霉菌F46和生防放线菌SC11进行原生质体融合,通过菌落形态特征及颜色的差异分离获得8株融合菌株。皿内实验结果表明,包括F-S5,F-S8,F-S12,F-S15和F-S16在内的5个菌株保留了亲本 SC11较强的抑菌作用,其中F-S8只对粉红聚端孢(Trichothecium roseum)有较强的抑菌活性,抑菌率>60％;F- S12对粉红聚端孢(T.roseum)和大丽轮枝菌(Verticillium dahliae)有抑菌作用,抑菌率接近60％;而其他3个菌株对5种靶标病原真菌皆有强的抑菌活性,抑菌率可达70％左右。在受抑菌丝再生能力测定中发现,菌株F-S15对粉红聚端孢(T.roseum)有永久抑菌作用;菌株F-S5对胶胞炭疽菌(Colletotrichum gloeosporioides)以及菌株F-S15对大丽轮枝菌(V．dahliae)的永久抑菌作用比较显著。显微观察发现,受融合菌株抑制的菌丝均有不同程度的畸变。

The protoplast fusion between hyperparasitic Streptomyces strain F46 and biocontrol actinomycetes strain SC11 was Studied and eight recombinant strains were isolated based on the morphological characteristics and colour of colony. The strong inhibiting effects in vitro of strain SCll on the target pathogens were inherited by five recombinant strains F-S5,F-S8,F-S12,F-S15 and F-S16. The strain F-S8 had obvious inhibiting effects （〉60%） on Trichothecium roseum and strain F-S12 had nearly the same effects on T. roseum and Verticillium dahliae. Strain F-S15 had nearly permanent inhibiting effects on T. roseum ;The strong permanent inhibiting effects were observed on strain F-S5 to Colletotrichum gloeosporioides and strain F-S15 to V. dahliae. The malformed and/or lysis mycelia of target pathogens were found when affected with the recombinant strains.