摘要
了解博卡病毒(Human Bocavirus,HBoV)在我国儿童急性呼吸道疾病中的感染情况。采用PCR扩增的方法对2005年10月~2006年1月收集的72例急性呼吸道感染的住院儿童鼻咽抽吸物(nasopharyngeal aspirates,NPA)进行了HBoV基因检测。将PCR阳性产物进行TA克隆,测序,并将所测序列与GenBank中HBoV序列进行比较分析。72份标本中共检测到6份HBoV阳性扩增产物,阳性率为8.3%(6/72),该6例HBoV刚性患儿临床均有肺炎或支气管肺炎症状。由此可以初步看出HBoV可能也是儿童急性呼吸道感染中较为重要的一个病原,且可能与儿童急性下呼吸道感染存在相关性。
In order to understand the epidemic of human boeavirus (HBoV) in China, PCR assay was applied to investigate HBoV infection among hospitalized children with acute respiratory tract disease (ARTI) from October 15, 2005 to January 8, 2006.Samples of nasopharyngeal aspirates(NPA) were collected and nucleic acids were extracted. Six of 72 samples(8.3 % ) were tested positive for HBoV by PCR. The PCR products were separately linked with T-vector and cloned, sequenced. Six gene sequences were compared and analyzed with those in GenBank. All these HBoV- infected patients had clinical symptoms of pneumonia. From above, we can conclude tentatively that HBoV may be an important pathogen in children with ARTI and probably has a correlation with acute lower respiratory tract infection of the children. This is the first report on HBoV in China.
出处
《病毒学报》
CAS
CSCD
北大核心
2006年第2期79-82,共4页
Chinese Journal of Virology