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人LNα4LG4-5在毕赤酵母中的表达研究

Expression of Human LNα4LG4-5 in Pichia Yeast
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摘要 为实现人层粘连蛋白α4链LG4-5组件(Human Laminin Alpha4Chain LG4-5Module,hLNα4LG4-5)蛋白的分泌表达,采用DNA重组技术将hLNα4LG4-5cDNA片段插入分泌型酵母表达载体pPICZαA中,构建了相应的重组表达质粒pPICZαA-LG45并在GS115毕赤酵母菌株中表达,纯化蛋白后进行细胞学实验证明,hLNα4LG4-5有明显促进肿瘤细胞粘附和扩展的作用,为深入研究LG4-5组件结构与功能奠定了基础。 To carry out the secretive expression of human laminin alpha4 chain LG4-5 module （hLNα4LG4-5）, recombinant expression plasmid pPICZαA-LG45 was constructed by inserting of hLNα4LG4-5 cDNA into yeast expression vector pPICZαA. The hLNα4LG4-5 protein was expressed in GS115 Pichia yeast strain after induced by methanol, and purified target protein can obviously promote the expansion and adhesion of 293 cells.
作者 连继勤 戴旭芳 李晓辉 何凤田
机构地区 第三军医大学基础医学系 重庆师范大学特殊教育学院
出处 《生物工程学报》 CAS CSCD 北大核心 2006年第2期226-230,共5页 Chinese Journal of Biotechnology
基金 国家自然科学基金资助项目(No.30400559)。~~
关键词 hLNα4LG4—5 Pichia yeast 基因表达 hLNα4LG4-5, Pichia yeast, gene expression
分类号 Q786 [生物学—分子生物学]
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参考文献11

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二级参考文献6

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生物工程学报
2006年 第2期

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