共生小球藻对绿草履虫盐耐受性及同工酶的影响

Effects of Symbiont on Saline Tolerance and Isozymes of Host in Paramecium bursaria-Chlorella System

为探索共生小球藻对宿主绿草履虫细胞生理和代谢过程的影响,比较了含共生藻绿草履虫(CCP)、无共生藻绿草履虫(CFP)和恢复型绿履草虫(RCCP)对NaCl或Pb(NO3)2的耐受性,分析了三者ATP酶同工酶等12种同工酶的活性和组分.其中CCP和RCCP氯化钠耐受性相似,且均不同于CFP;CFP与RCCP在硝酸铅耐受性及同工酶构成和酶活性上有较多相似性.RC-CP的同工酶活性和组分的特征介于CCP与CFP两者之间.CCP中同工酶组分减少和酶活性降低的现象提示,绿草履虫-小球藻共生系统中,小球藻的共生作用可能会促使宿主酶系的退化.

To further investigate the effect of symbiotic Chlorella on physiological process and metabolism of the host P. bursaria, NaCl or Pb（NO3）2 tolerance and 12 types of isozyme of Chlorella containing P. bursa ria（CCP） and recovered Chlorella-containing P. bursaria（RCCP） and Chlorella-free P. bursaria（CFP） were analyzed. The results showed that NaCl tolerance of CCP was similar to that of RCCP, different from that of CFP, and that Pb（NO3）2 tolerance and activity of isozymes of CFP were similar to those of RCCP. RCCP showed the transitional charaeteristics between CCP and CFP. Diverse composition and activity of isozymes of among the three samples indicated existence or symbiotic time of Chlorella could change the isozyme system of the host. Fewer composition and lower activity of isozyme in CCP probably implied that due to the symbiosis of Chlorella, isozyme system of the host degenerated in ＂P. bursaria-Chlorella＂ system.