TNF-α基因修饰的口腔鳞癌DNL生物学及免疫学特性研究

Experimental study on biological and immunological characteristics of modified DNL of oral squamous cell carcinoma with TNF-α gene

作者观察了转导及未转导ＴＮＦ－α。基因的ＤＮＬ在含ｒＩＬ－２培养基中，以及转导基因ＤＮＬ在不含ｒＩＬ－２培养基中的生长特性；并应用流式细胞仪对转导及未转导基因ＤＮＬ的ＤＮＡ指数、细胞周期、免疫表型进行了分析，结果显示：转导及未转导基因ＤＮＬ体外生长情况基本一致，转导基因ＤＮＬ在无ｒＩＬ－２培养基中无自主性生长；转导及未转导基因ＤＮＬ的ＤＮＡ指数、细胞周期时相分布及免疫表型完全一致．这一结果表明，转导ＴＮＦ－α基因对ＤＮＬ的生物学及免疫学特性无影响。

Growing characteristic of gene-transduced and nontransduced DNL in medium of containing rIL-2 was compared, and growing characteristic of gene-transduced DNL in medium of non containing rIL-2 was analyzed. DNA index (DI), cell cycle and im munologic phenotypes of gene-transduced and nontransduced DNL were analyzed with FACS. The results revealed introducing TNF-αgene into DNL was not interfered with growth,DI ,cell cycle and immunologic phenotypes of DNL, and gene-transduced DNL couldn't growing infinitely.