摘要
作者观察了转导及未转导TNF-α。基因的DNL在含rIL-2培养基中,以及转导基因DNL在不含rIL-2培养基中的生长特性;并应用流式细胞仪对转导及未转导基因DNL的DNA指数、细胞周期、免疫表型进行了分析,结果显示:转导及未转导基因DNL体外生长情况基本一致,转导基因DNL在无rIL-2培养基中无自主性生长;转导及未转导基因DNL的DNA指数、细胞周期时相分布及免疫表型完全一致.这一结果表明,转导TNF-α基因对DNL的生物学及免疫学特性无影响。
Growing characteristic of gene-transduced and nontransduced DNL in medium of containing rIL-2 was compared, and growing characteristic of gene-transduced DNL in medium of non containing rIL-2 was analyzed. DNA index (DI), cell cycle and im munologic phenotypes of gene-transduced and nontransduced DNL were analyzed with FACS. The results revealed introducing TNF-αgene into DNL was not interfered with growth,DI ,cell cycle and immunologic phenotypes of DNL, and gene-transduced DNL couldn't growing infinitely.
出处
《上海口腔医学》
CAS
CSCD
1996年第1期13-16,共4页
Shanghai Journal of Stomatology
基金
国家八六三课题基金
国家自然科学基金资助课题