摘要
目的:建立高效液相色谱法测定血浆中法舒地尔的浓度。方法:色谱柱:Hyperail C18(4．6mm×250 mm,10μm);流动相:0．05 mol·L-1磷酸二氢钾(pH 5．0)-甲醇(75:25);流速:1．0 ml·min-1;柱温:40℃;检测波长:276 nm。结果:法舒地尔在10-1000 ng·ml-1浓度范围内线性关系良好(r=0．999 1);低、中、高三种浓度相对回收率分别为89．73％,103．75％, 104．57％:日内、日间RSD均小于10％。结论:本法可行。
Objective： To establish an HPLC method for the determination of fasudil in blood plasma. Method： the Hypersil C18 column （4.6 mm× 250 mm, 10μm） was used. The mobile phase was 0.05 mol· L^-1 potassium dihydrogen phosphate-methanol （75 ： 25）. The flow rate was 1.0 ml· min^-1.The column temperature at 40 ℃ the Detection wavelength was at 276 nm. Result： The linear range of fasudil was 10 - 1000μg·L^-1 （r =0. 999 1 ） ,The recoveries were 84.86% ,101.51% ,104.57% in the low,middle and high concentrations, respectively and RSD were less than 10%. Conclusion： The method can be used for the determination the content of fasudil in blood plasma.
出处
《中国药师》
CAS
2006年第4期316-317,323,共3页
China Pharmacist
