CD44v6、nm23及基质金属蛋白酶9在胃癌组织中的表达及其意义

Expression and significance of CD44v6 and nm23 and MMP-9 in gastric cancer

目的探讨CD44v6、nm23及基质金属蛋白酶9(MMP-9)在胃癌组织中的表达及其与胃癌浸润和转移等临床病理因素间的关系。方法应用免疫组织化学S-P法观察并比较70例胃癌、20例慢性浅表性胃炎、20例伴大肠上皮化生的慢性萎缩性胃炎及20例伴异型增生的慢性萎缩性胃炎组织中CD44v6、nm23和MMP-9的表达情况。结果CD44v6、nm23和MMP-9在胃癌中的阳性表达率分别为64.3%、60.0%及57.1%。胃癌CD44v6和MMP-9表达率高于对照组,nm23表达率低于对照组。CD44v6、MMP-9和nm23在有、无淋巴转移组织中的表达差异均有统计学意义;MMP-9和nm23在有、无浆膜浸润组织中的表达差异有统计学意义。CD44v6、nm23、MMP-9在胃癌组织中的表达具有相关性。结论检测CD44v6、MMP-9及nm23在胃癌中的表达有助于胃癌恶性程度、浸润深度及淋巴结转移的判断以及患者预后的评估。

Objective To investigate the relationship among the expressions of CD44v6,nm23 and MMP-9 and lymph node metastasis,differentiated degree as well as invasion depth in primary gastric carcinoma. Methods Seventy cases of gastric carcinoma,20 cases of chronic superficial gastritis,20 cases of large intesinal metaplasia and 20 cases of atipical hyperplasia were investigated using antibodies to CD44v6, MMP-9 and nm23 by S-P immunohistochemical technique. Results The positivity rates of CD44v6, MMP-9 and nm23 in gastric cancer were 64.3 %, 60.0 %, 57.1%, respectively. The positivity rates of CD44v6 and MMP-9 in gastric carcinoma were higher than that in the control groups. The positivity rates of nm23 in gastric carcinoma were lower than that in the control groups. The difference between the expression of CD44v6, MMP-9 and nm23 and lymph node metastasis was statistically significant. There was statistically significant difference between the expression of MMP-9, nm23 and the depth of invasion. CD44v6 expression in gastric cancer was positively correlated with MMP-9 expression（ P 〈 0.01 ）, but neg- atively correlated with nm2.3 expression（ P 〈 0.05 ）. nm2.3 expression in gastric cancer was negatively correlated with MMP-9 expression（P 〈 0.01 ）. Conclusion The detection of CD44v6, MMP-9 and nm23 is valuable in evaluating differential degree, depth of invasion and lymph node metastasis of gastric cancer.