慢性肝病患者肝细胞生长因子的测定及意义

Role of Hepatocyte growth factor in chronic liver disease

目的探讨血清肝细胞生长因子(HGF)水平在慢性肝病中的改变及意义。方法用ELISA法检测肝细胞癌(HCC)50例,肝硬化(LC)59例,慢性肝炎(CH)39例,正常对照36例的HGF的水平,并描绘受试者工作曲线(ROC),确定HCC和LC患者HGF水平的最佳临界点。运用Spearman相关分析观察HGF水平与ALT、AST、白蛋白、总胆红素、凝血时间、肝癌大小、病理分级的相关性。结果HCC、LC、CH和正常对照组的血清HGF含量分别是(45.64±40.68)pg/ml、(630.84±469.12)pg/ml、(59.24±36.13)pg/ml和(8.02±5.51)pg/ml;HCC组和LC组的血清HGF水平显著高于正常对照组(t=2.794,t=2.851,P<0.01);LC组Child C级患者的HGF水平明显高于Child A、B级(t=2.890,t=3.091,P<0.01);血清HGF水平仅发现与凝血时间有相关性(r=0.45,P<0.01),与肿瘤大小、病理分级无关。结论血清中HGF水平增高与LC程度有关。

Objective To discussed the significance of serum Hepatocyte growth factor（HGF） in chronic liver diseases. Methods 50 cases with hepatoeellular carcinoma（HCC）, 59 cases with liver cirrhosis（LC）, 38 cases with chronic hepatitis（CH） and 36 healthy people were detected by enzyme linkedimmunosorbent assay（ELISA）. Results The serum HGF levels of patients of HCC, LC, CH and normal control were （45.64 ± 40.68） pg/ml, （630.84 ± 469.12 ） pg/ml, （59.24 ± 36.13 ） pg/ml, （ 8.02 ± 5.51 ） pg/ml, espectively. Serum HGF levels of HCC and LC patients were significantly higher than those of healthy group（ t = 2. 794, t = 2.851, P 〈 0.01 ）. Patients of LC with Child C had higher HGF levels than those with Child A or B（ t = 2. 890, t = 3. 091, P 〈 0.01 ）. In LC group, the ROC curve suggested that the perfect HGF cut off point should be set at 14pg/ml to gain the best sensitivity and specificity. Serum HGF level was correlated with prothrombin time（ r = 0.45, P〈 0.01 ）. No correlation was found between serum HGF and tumor size or tumor pathological grade. Conclusion The expression of HGF may be correlated with LC.